Summary Project

Peter Agre was awarded one half of the Nobel Prize in chemistry 2003 for discoveries concerning channels in cell tissue layers.
Water is a study component of all our cells. Multicellular organisms face a enigma of how to transport fluids across biological barriers. Moreover, researchers have found that body of water permeability can be heightened in tissues corresponding nephritic tubule, secretory glands, so simple diffusion cannot be the centre explanation for fluid transport. Technical problems withal hinder the come of the field. Firstly, water is everywhere and not amenable to chemical modifications like chemical cross-linking groups or labels. Secondly, expression cloning to isolate the proteins turn out challenging due(p) to its identity as a membrane protein. Also relatively game permeability of membrane bilayers kick in to a high background. Lastly technology advancements aided the high resolution strucutral elucidation of proteins only lately.

Agre discovered the AQP 1 by serendipity during his research of Rh blood group antigens. The contaminant obscure from antibody-binding was a novel protein that was tetrameric membrane-spanning protein and abundant in areas which are passing permeable to water. Agre confirmed its function using frog oocytes due to their low water permability.



Only oocytes with AQP1 experienced cytolysis when placed in water. Next Agre elucidated the structure using various methods like hydrophathy plots, hitch fracture EM, X ray crystallography and model-making via site-directed mutagenisis studies etc. He also found the fundamental link between structure and function. AQP1 is a 6 TM protein that forms tetramers, with each monomer forming a single channel. curl up B and E forms a pore via NPA-motif juxtaposition, thus forming an hourglass model. The contriction at the middle of the hourglass is about 20 Angstrom, allowing single-file movement of watermolecules as H bonds can be eliminated via transient interaction with pore residues. Protons...If you urgency to get a full essay, order it on our website: Ordercustompaper.com



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